بانک مقالات

مهندسی متابولیک Yarrowia lipolytica برای تولید مواد شیمیایی و سوخت از xylose

Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose

سال انتشار : 2016

تعداد صفحه : 10

کلیدواژه ها:  Yarrowia lipolytica, Xylose, Microbial oils, Metabolic engineering, Lignocellulosic materials, Synthetic biology

   دانلود    سفارش ترجمه

ABSTRACT

Yarrowia lipolytica is a biotechnological chassis for the production of a range of products, such as microbial oils and organic acids. However, it is unable to consume xylose, the major pentose in lignocellulosic hydrolysates, which are considered a preferred carbon source for bioprocesses due to their low cost, wide abundance and high sugar content. Here, we engineered Y. lipolytica to metabolize xylose to produce lipids or citric acid. The overexpression of xylose reductase and xylitol dehydrogenase from Scheffersomyces stipitis were necessary but not sufficient to permit growth. The additional overexpression of the endogenous xylulokinase enabled identical growth as the wild type strain in glucose. This mutant was able to produce up to 80 g/L of citric acid from xylose. Transferring these modifications to a lipid-overproducing strain boosted the production of lipids from xylose. This is the first step towards a consolidated bioprocess to produce chemicals and fuels from lignocellulosic materials.

زبان فایل

مجلات و ژورنال های بین المللی

Elsevier

ترجمه

نوع فایل

رایگان

سال انتشار

2016

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